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Thowing Plasmodium berghei

Posted by on December 30, 2013

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  1. Disuhu ruangkan sampai sampel darah mencair
  2. Diambil sampel dimasukkan pada falcon 15 mL
  3. Disentrifuse 2000rpm selama 5 menit
  4. Dibuang supernatannya
  5. Pelet dicuci dengan 10 mL PBS atau 2% FBS dalam PBS
  6. Disentrifuse 2000 rpm selama 10 menit
  7. Dibuang supernatan
  8. Diulangi prosedur 6-8 sebanyak 2x
  9. Pelet ditambah 1mL medium komplet (RPMI +)
  10. Diambil dengan spuit 1 mL
  11. Disuntikkan pada mencit sebanyak 200 ┬ÁL

1. ip mencit

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